CTS Reagents
The Collaborative Transplant Study provides high quality serological and molecular HLA-typing reagents and kits for antibody testing to study participants at self-cost. These reagents have been available for many years and we believe that they are an important contributing factor to the excellent correlation of immunological parameters with graft survival found in the CTS data. The CTS website contains the current versions of kit manuals as well as updated interpretation schemes and tables. The manuals are available in PDF-format. To display PDF image format, Adobe´s freely available Acrobat Plug-in or Acrobat Reader Software is required.
Each product can be identified by its 3-digit ordering code (product number).
The CTS-PCR-SSP reagents have received CE-Certificate in accordance with the requirements of the In Vitro Diagnostic Directive 98/79/EC. The Quality Management System is EN ISO 13485:2003 certified.
see also: CE Certificate, CE Certificate / Attachment, ISO 9001 Certificate, ISO 13485 Certificate
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| HLA Sequencing-Based Typing with CTS-SEQUENCE Kits for HLA class I and class II |
CTS-SEQUENCE Kits are designed to provide easy-to-use and cost-effective HLA high resolution typing.
- Achieving the "golden mean": The use of a minimum of allele-group specific mixes (amplifying the most relevant allele groups) in combination with locus-specific mixes allows separation of the haplotypes, minimizing the number of ambiguities and the risk of allele-dropout. At the same time, the low number of PCRs ensures an economical way of testing.
- The combination of forward and reverse sequencing reactions enables complete and accurate identification of all sequences. Exons 2, 3 and 4 of HLA class I, Exon 2 and 3 of HLA-DQB1, and Exon 2 of HLA-DRB1 are covered.
- Uniform protocol for all HLA loci eases use for flexible throughput needs.
- Sequence analysis can be performed with the software Sequence Pilot (JSI medical systems, Germany) or other HLA analysis software readily available in your lab. Alternatively, you can send us your electropherograms for allele assignment.
Molecular HLA Typing Reagents
PCR-SSP reagents for HLA class I and class II typing are available in three forms:
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Tray Kits
Tray Kits consist of 96-well thin-walled PCR trays. A Mastermix (PCR reaction buffer) is provided. Each well contains a different lyophilized PCR primer mix. Simply add DNA, Mastermix and Taq polymerase.
Main Manual for Tray and Minitray Kits #100A (PDF)
MSDS for Tray and Minitray Kits #100B (PDF)
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Minitray Kits
Minitray Kits consist of either 16-, 24- or 32-well thin-walled PCR trays. A Mastermix (PCR reaction buffer) is provided. Each well contains a different lyophilized PCR primer mix. Simply add DNA, Mastermix and Taq polymerase.
Main Manual for Tray and Minitray Kits #100A (PDF)
MSDS for Tray and Minitray Kits #100B (PDF)
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Bulk Reagents
Sets of PCR mixes aliquoted in bulk tubes for 100 typings.
Main Manual for Bulk Kits #400A (PDF)
MSDS for Bulk Kits #400B (PDF)
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CTS Sequence Kits
Molecular Cytokine Typing Kits
Prealiquoted PCR-SSP reagents for cytokine gene polymorphism typing.
Molecular HPA Typing Kits
Prealiquoted PCR-SSP reagents for HPA gene polymorphism typing.
Molecular Minor Histocompatibility Antigen Typing Kits
Prealiquoted PCR-SSP reagents for Minor Histocompatiblity Antigen polymorphism typing.
Serological Reagents for Antibody Screening and HLA Typing
Frozen lymphocyte trays for antibody screening and serum trays for serological HLA class I and class II typing.
CTS 4-ELISA Kits
- Allow the simultaneous testing of 21 human serum samples for 4 different immune parameters (sCD30, IgG-anti-HLA class I, IgG-anti-HLA class II, IgA-anti-Fab)
- 96-well strip format
- ELISA reader with 405 nm primary and 492 nm reference wavelength is required
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ELISA Kits
Manual (PDF)
Short Manual (PDF)
Other Products
Further Information
For more detailed information about CTS reagents please contact:
Name:Hien Tran, M.D.
Phone:+49 6221 564005
Email:hien.tran@med.uni-heidelberg.de