CTS - Collaborative Transplant Study

Reagents

CTS Reagents

The Collaborative Transplant Study provides high quality serological and molecular HLA-typing reagents and kits for antibody testing to study participants at self-cost. These reagents have been available for many years and we believe that they are an important contributing factor to the excellent correlation of immunological parameters with graft survival found in the CTS data. The CTS website contains the current versions of kit manuals as well as updated interpretation schemes and tables. The manuals are available in PDF-format. To display PDF image format, Adobe´s freely available Acrobat Plug-in or Acrobat Reader Software is required. (Obtain free Adobe Acrobat.)

Each product can be identified by its 3-digit ordering code (product number).

The CTS-PCR-SSP reagents have received CE-Certificate in accordance with the requirements of the In Vitro Diagnostic Directive 98/79/EC. The Quality Management System is EN ISO 13485 certified.

New PCR-SBT products:

HLA Sequencing-Based Typing
with
CTS-SEQUENCE Kits for HLA class I and class II

CTS-SEQUENCE Kits are designed to provide easy-to-use and cost-effective HLA high resolution typing.

Achieving the "golden mean": The use of a minimum of allele-group specific mixes (amplifying the most relevant allele groups) in combination with locus-specific mixes allows separation of the haplotypes, minimizing the number of ambiguities and the risk of allele-dropout. At the same time, the low number of PCRs ensures an economical way of testing.
The combination of forward and reverse sequencing reactions enables complete and accurate identification of all sequences. Exons 2, 3 and 4 of HLA class I, Exon 2 and 3 of HLA-DQB1, and Exon 2 of HLA-DRB1 are covered.
Uniform protocol for all HLA loci eases use for flexible throughput needs.
Sequence analysis can be performed with the software Sequence Pilot (JSI medical systems, Germany) or other HLA analysis software readily available in your lab. Alternatively, you can send us your electropherograms for allele assignment.

Two new PCR-SSP Kits were developed for the distinction of the following common and well-documented alleles:

HLA-C*04:01/ HLA-C*04:09N
HLA-DRB1*14:01/ HLA-DRB1*14:54

Molecular HLA Typing Reagents

PCR-SSP reagents for HLA class I and class II typing are available in three forms:

Tray Kits (Click link for locus-specific kits)
Tray Kits consist of 96-well thin-walled PCR trays. A Mastermix (PCR reaction buffer) is provided. Each well contains a different lyophilized PCR primer mix. Simply add DNA, Mastermix and Taq polymerase.
Main Manual for Tray and Minitray Kits #100A (PDF)
MSDS for Tray and Minitray Kits #100B (PDF)
Minitray Kits (Click link for locus-specific kits)
Minitray Kits consist of either 8-, 16-, 24- or 32-well thin-walled PCR trays. A Mastermix (PCR reaction buffer) is provided. Each well contains a different lyophilized PCR primer mix. Simply add DNA, Mastermix and Taq polymerase.
Main Manual for Tray and Minitray Kits #100A (PDF)
MSDS for Tray and Minitray Kits #100B (PDF)

PCR-SBT reagents for HLA class I and class II typing:

CTS Sequence Kits (Click link for locus-specific kits)

Molecular Cytokine Typing Kits

Prealiquoted PCR-SSP reagents for cytokine gene polymorphism typing.

Tray Kits (Click link for lot-specific kits)

Molecular HPA Typing Kits

Prealiquoted PCR-SSP reagents for HPA gene polymorphism typing.

Minitray Kits (Click link for lot-specific kits)

Serological Reagents for Antibody Screening and HLA Typing

Frozen lymphocyte trays for antibody screening and serum trays for serological HLA class I and class II typing.

Other Products

Further Information

For more detailed information about CTS reagents please contact:

Name:Hien Tran, M.D.
Phone:+49 6221 564005
Email:hien.tran@med.uni-heidelberg.de

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